Catalog Number - 40231-05P-2

40231-05P-2 Cogent UDA™ (weak cation exchange, WCX) HPLC Column is a highly specialized stationary phase built on Cogent TYPE‑C™ silica (silica hydride), delivering unique and powerful selectivity not achievable with traditional silica phases such as C18 or standard ion exchange columns.

The UDA™ ligand (Undecanoic Acid) consists of an 11‑carbon chain with a terminal carboxylic acid group, providing multi‑mode retention behavior:

• Reversed phase (hydrophobic interactions)

• Weak cation exchange (via ionizable acid group)

• HILIC (polar interaction capability)

This combination allows enhanced separation of polar, basic, ionizable, and structurally similar compounds, particularly in complex matrices where traditional methods fail.

Unlike conventional silica columns, the TYPE‑C silica platform provides:

• Faster equilibration

• Greater robustness

• Reduced sensitivity to mobile phase water content

50 mm Length – Fast High‑Efficiency LC‑MS Workflows

The 50 mm length is optimized for speed and efficiency in modern UHPLC-style workflows.

• Very fast analysis times

• Reduced solvent consumption

• Ideal for method development and screening

✅ Recommended for LC‑MS methods, rapid separations, and high‑throughput workflows

Flow Rate & System Requirements (2.1 mm ID, 2.2 µm)

⚠️ Requires UHPLC or low‑flow capable HPLC system

• Typical flow rate range: ~0.2 – 0.4 mL/min

• Higher backpressure due to 2.2 µm particles

• Requires precise, low‑volume pumping capability

• Optimized for low‑dispersion flow paths and small detector volumes

✅ Important: Standard HPLC systems must be capable of stable and accurate low‑flow delivery

✅ System Fit: Ideal for LC‑MS and UHPLC systems with low delay volume

Unique Selectivity – Why Choose UDA™ (WCX)

Mixed-Mode Retention Mechanisms

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Complex Matrices

• Resolves co‑eluting and structurally similar compounds

• Separates ionizable analytes effectively

pH‑Driven Selectivity Control

• WCX mode active at pH ≥ 6

• Tunable retention using pH and ionic strength

✅ Ideal when C18, phenyl, or standard ion exchange columns fail

TYPE‑C Silica Advantages

Faster Equilibration

• Reduces downtime between runs

No Strict Moisture Control Required

• More forgiving than traditional HILIC columns

Robust Column Lifetimes

• Stable chemistry for extended use

Low Salt Requirements (HILIC Mode)

• Easier MS compatibility

• Cleaner baselines

Cogent UDA™ Column Advantages

True Multi‑Mode Selectivity

• Combines RP, HILIC, and WCX in one column

Near UHPLC Performance (2.2 µm)

• High efficiency with manageable pressure

Enhanced LC‑MS Compatibility

• Lower salt requirements vs traditional ion exchange

Typical Applications

• Complex matrix separations

• LC‑MS method development

• Polar and ionizable compound analysis

• Basic compound retention and resolution

• Pharmaceutical and bioanalytical workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Requires UHPLC or low‑flow capable HPLC system

• Optimized for low volume, high efficiency systems

• pH control is critical for WCX functionality